Anti-TMPO Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P42166\|P42167
Gene Id	7112
Gene Symbols	TMPO
Protein Name / Synonyms	TMPO, CMD1T, LAP2, LEMD4, PRO0868, TP, thymopoietin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

38kDa/50kDa/75kDa

Observed MW

56kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TMPO antibody (144-02534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] TMPO Polyclonal Antibody (144-02534)

Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (144-02534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - [KO Validated] TMPO Polyclonal Antibody (144-02534)

Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (144-02534) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (144-02534)

Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (144-02534) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (144-02534)

Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (144-02534) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (144-02534)

Immunofluorescence analysis of U2OS cells using TMPO antibody (144-02534).
Immunofluorescence - [KO Validated] TMPO Polyclonal Antibody (144-02534)

Expiration:

12 months from the date of shipment when stored properly.

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