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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P01375 |
| Gene Id | 7124 |
| Gene Symbols | TNF |
| Protein Name / Synonyms | TNF, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Dilutions
WB 1:1000 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
25kDa
Observed MW
21kDa
Immunogen
A synthetic peptide of human TNF
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TNF-? antibody (144-011534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using TNF-? antibody (144-011534) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of RAW264.7 cells using TNF-? antibody (144-011534) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.