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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O00300 |
| Gene Id | 4982 |
| Gene Symbols | TNFRSF11B |
| Protein Name / Synonyms | TNFRSF11B, OCIF, OPG, PDB5, TR1, TNF receptor superfamily member 11b |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
46kDa
Observed MW
44kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-280 of human TNFRSF11B (NP_002537.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TNFRSF11B antibody (144-02100) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human gastric cancer using TNFRSF11B Antibody (144-02100) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using TNFRSF11B Antibody (144-02100) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using TNFRSF11B Antibody (144-02100) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TNFRSF11B Antibody (144-02100) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using TNFRSF11B Antibody (144-02100) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using TNFRSF11B Antibody (144-02100) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using TNFRSF11B Antibody (144-02100) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using TNFRSF11B antibody (144-02100). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.