TNNI3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P19429 |
| Gene Id | 7137 |
| Gene Symbols | TNNI3 |
| Protein Name / Synonyms | TNNI3, CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I3, cardiac type |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
24kDa
Observed MW
26kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TNNI3 (NP_000354.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TNNI3 antibody (144-06995) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human stomach using TNNI3 antibody (144-06995) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.