TOLLIP Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9H0E2 |
| Gene Id | 54472 |
| Gene Symbols | TOLLIP |
| Protein Name / Synonyms | TOLLIP, IL-1RAcPIP, toll-interacting protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
22kDa/30kDa
Observed MW
30kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human TOLLIP (NP_061882.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TOLLIP antibody (144-02202) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human stomach using TOLLIP antibody (144-02202) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TOLLIP antibody (144-02202) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using TOLLIP antibody (144-02202) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using TOLLIP antibody (144-02202) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TOLLIP antibody (144-02202) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TOLLIP antibody (144-02202). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.