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Anti-TOMM20 Antibody
Catalog #: 144-06774
TOMM20 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15388 |
| Gene Id | 9804 |
| Gene Symbols | TOMM20 |
| Protein Name / Synonyms | TOMM20, MAS20, MOM19, TOM20, translocase of outer mitochondrial membrane 20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:20 - 1:50
Format
Calculated MW
16kDa
Observed MW
16kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOMM20 (NP_055580.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TOMM20 antibody (144-06774) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver cancer using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using TOMM20 antibody (144-06774). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using TOMM20 antibody (144-06774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.