Anti-TOP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P11387
Gene Id	7150
Gene Symbols	TOP1
Protein Name / Synonyms	TOP1, TOPI, DNA topoisomerase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50

Format

Calculated MW

90kDa

Observed MW

120kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP1 (NP_003277.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TOP1 antibody (144-12524) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - TOP1 Polyclonal Antibody (144-12524)

Immunohistochemistry of paraffin-embedded mouse liver using TOP1 antibody (144-12524) at dilution of 1:100 (40x lens).
Immunohistochemistry - TOP1 Polyclonal Antibody (144-12524)

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug TOP1 antibody (144-12524). Western blot was performed from the immunoprecipitate using TOP1 antibody (144-12524) at a dilition of 1:1000.
Immunoprecipitation - TOP1 Polyclonal Antibody (144-12524)

Expiration:

12 months from the date of shipment when stored properly.

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