Anti-TOP1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P11387
Gene Id	7150
Gene Symbols	TOP1
Protein Name / Synonyms	TOP1 (N-term)
Clonality	Polyclonal
Preparation	This TOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human TOP1.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Specificity

Predicted Reactivity

Mouse, Rat, Hamster

Other Names

TOP1; DNA topoisomerase 1; DNA topoisomerase I

NCBI Accession #

NP_003277.1

Other Accession #

Q9WUL0; Q04750; Q07050; NP_003277.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

90726

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	TOP1 Antibody (N-term) (Cat# 102-12208) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TOP1 antibody detected the TOP1 protein (arrow).
	Confocal immunofluorescent analysis of TOP1 Antibody (N-term)(Cat# 102-12208) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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