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Anti-TOP2A / Topoisomerase II alpha Antibody
Catalog #: 188-11199
Mouse anti-Human/Mouse TOP2A Antibody / Topoisomerase II alpha
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Mouse |
| Accession Number | P11388 |
| Gene Id | 7153 |
| Gene Symbols | TOP2A,TOP2 |
| Protein Name / Synonyms | DNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme) |
| Immunogen | Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody. |
| Isotype | Kappa, IgG2b |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | FACS: 0.5-1ug/10^6 cells in 0.1ml IF: 1-2ug/ml Western blot: 1-2ug/ml IHC (FFPE): 0.5-1ug/ml |
| Domain / Region / Terminus | Nuclear |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Storage
Store the Topoisomerase II alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.