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Anti-TP53 Antibody
Catalog #: 144-05761
TP53 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 22059 |
| Gene Symbols | TP53 |
| Protein Name / Synonyms | TP53, BCC7, LFS1, P53, TRP53, tumor protein p53 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP, ChIP |
| Research Area | Cancer, p53 Signaling, DNA Damage |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Dilutions
WB 1:3000 - 1:6000
IHC 1:50 - 1:200
IP 1:20 - 1:50
ChIP 1:20 - 1:50
IHC 1:50 - 1:200
IP 1:20 - 1:50
ChIP 1:20 - 1:50
Format
Calculated MW
43kDa
Observed MW
53kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 218-390 of mouse TP53 (NP_035770.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TP53 antibody (144-05761) at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat ovary using TP53 antibody (144-05761) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TP53 antibody (144-05761) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using TP53 antibody (144-05761) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TP53 antibody (144-05761) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug TP53 antibody (144-05761). Western blot was performed from the immunoprecipitate using TP53 antibody (144-05761) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.