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Anti-TP53BP1 Antibody
Catalog #: 144-05757
TP53BP1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12888 |
| Gene Id | 7158 |
| Gene Symbols | TP53BP1 |
| Protein Name / Synonyms | TP53BP1, 53BP1, TDRD30, TP53, p202, p53BP1, tumor protein p53 binding protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
213kDa/214kDa
Observed MW
255kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human TP53BP1 (NP_001135452.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of A-431 cells, using TP53BP1 antibody (144-05757) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using TP53BP1 antibody (144-05757) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TP53BP1 antibody (144-05757) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TP53BP1 antibody (144-05757) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using TP53BP1 antibody (144-05757) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using TP53BP1 antibody (144-05757) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TP53BP1 antibody (144-05757) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TP53BP1 antibody (144-05757). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using TP53BP1 antibody (144-05757). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Expiration:
12 months from the date of shipment when stored properly.