TRAP1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12931 |
| Gene Id | 10131 |
| Gene Symbols | TRAP1 |
| Protein Name / Synonyms | TRAP1, HSP 75, HSP75, HSP90L, TRAP-1, TNF receptor associated protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
74kDa/80kDa
Observed MW
82kDa
Immunogen
Recombinant protein of human TRAP1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TRAP1 antibody (144-02748) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody (144-02748) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat skeletal muscle using TRAP1 Antibody (144-02748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody (144-02748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRAP1 Antibody (144-02748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TRAP1 Antibody (144-02748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TRAP1 Antibody (144-02748) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TRAP1 antibody (144-02748). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.