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Anti-TRIM25 Antibody
Catalog #: 144-12938
TRIM25 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14258 |
| Gene Id | 7706 |
| Gene Symbols | TRIM25 |
| Protein Name / Synonyms | TRIM25, EFP, RNF147, Z147, ZNF147, tripartite motif containing 25 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
70kDa
Observed MW
68kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM25 (NP_005073.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TRIM25 antibody (144-12938) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug TRIM25 antibody (144-12938). Western blot was performed from the immunoprecipitate using TRIM25 antibody (144-12938) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.