Anti-TRIM28 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13263
Gene Id	10155
Gene Symbols	TRIM28
Protein Name / Synonyms	TRIM28, KAP1, PPP1R157, RNF96, TF1B, TIF1B, tripartite motif containing 28
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Dilutions

WB 1:1000 - 1:3000
IHC 1:100 - 1:200

Format

Calculated MW

79kDa/88kDa

Observed MW

120kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 576-835 of human TRIM28 (NP_005753.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TRIM28 antibody (144-02245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - TRIM28 antibody (144-02245)

Immunohistochemistry of paraffin-embedded rat brain using TRIM28 antibody (144-02245) at dilution of 1:100 (40x lens).
Immunohistochemistry - TRIM28 antibody (144-02245)

Expiration:

12 months from the date of shipment when stored properly.

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