Anti-TriMethyl-Histone H3-K14 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q16695
Gene Id	8290
Gene Symbols	H3K14ME3
Protein Name / Synonyms	HIST3H3, H3.4, H3/g, H3FT, H3t, histone H3.1t
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP, ChIP, ChIPseq
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100

Format

Calculated MW

15kDa

Observed MW

18kDa

Immunogen

A synthetic peptide of human TriMethyl-Histone H3-K14

Modification

TriMethyl K14

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K14 antibody (144-05279).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - TriMethyl-Histone H3-K14 antibody (144-05279)

Expiration:

12 months from the date of shipment when stored properly.