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Anti-TRIP12 Antibody
Catalog #: 144-09958
TRIP12 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14669 |
| Gene Id | 9320 |
| Gene Symbols | TRIP12 |
| Protein Name / Synonyms | TRIP12, TRIP-12, ULF, E3 ubiquitin-protein ligase TRIP12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53.
Dilutions
WB 1:500 - 1:1000
Format
Calculated MW
192kDa/220kDa/223kDa/225kDa
Observed MW
260kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human TRIP12 (NP_001271143.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TRIP12 antibody (144-09958) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.