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Anti-TRMT2A Antibody
Catalog #: 144-05975
TRMT2A Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8IZ69 |
| Gene Id | 27037 |
| Gene Symbols | TRMT2A |
| Protein Name / Synonyms | TRMT2A, HTF9C, tRNA methyltransferase 2 homolog A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 0.83 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
61kDa/68kDa
Observed MW
70kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human TRMT2A (NP_073564.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TRMT2A antibody (144-05975) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using TRMT2A antibody (144-05975) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRMT2A antibody (144-05975) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using TRMT2A antibody (144-05975) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TRMT2A antibody (144-05975) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using TRMT2A antibody (144-05975) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.