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Anti-TRMT2B Antibody

Catalog #: 144-03509

TRMT2B Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Rat
Host SpeciesRabbit
Accession Number
Q96GJ1
Gene Id
79979
Gene Symbols
TRMT2B
Protein Name / Synonyms
TRMT2B, CXorf34, dJ341D10.3, tRNA methyltransferase 2 homolog B
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms.

Dilutions

WB 1:1000 - 1:2000

Format

Calculated MW
51kDa/55kDa/56kDa
Observed MW
45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT2B (NP_079193.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TRMT2B antibody (144-03509) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - TRMT2B antibody (144-03509)

Expiration:
12 months from the date of shipment when stored properly.