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Anti-TSC22D3 Antibody
Catalog #: 144-06779
TSC22D3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q99576 |
| Gene Id | 1831 |
| Gene Symbols | TSC22D3 |
| Protein Name / Synonyms | TSC22D3, DIP, DSIPI, GILZ, TSC-22R, TSC22 domain family member 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor κB. Alternate splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
13kDa/14kDa/22kDa
Observed MW
15kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human TSC22D3 (NP_001015881.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TSC22D3 antibody (144-06779) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using TSC22D3 Antibody (144-06779) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using TSC22D3 Antibody (144-06779) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TSC22D3 Antibody (144-06779) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using TSC22D3 Antibody (144-06779) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TSC22D3 antibody (144-06779). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TSC22D3 antibody (144-06779). Western blot was performed from the immunoprecipitate using TSC22D3 antibody (144-06779) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.