Anti-TSEN2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8NCE0
Gene Id	80746
Gene Symbols	TSEN2
Protein Name / Synonyms	TSEN2, PCH2B, SEN2, SEN2L, tRNA splicing endonuclease subunit 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

45kDa/47kDa/49kDa/51kDa/53kDa

Observed MW

53kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TSEN2 (NP_079541.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TSEN2 antibody (144-07838) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - TSEN2 antibody (144-07838)

Immunofluorescence analysis of A549 cells using TSEN2 antibody (144-07838).
Immunofluorescence - TSEN2 antibody (144-07838)

Expiration:

12 months from the date of shipment when stored properly.

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