Anti-TSHR Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P16473
Gene Id	7253
Gene Symbols	TSHR
Protein Name / Synonyms	TSHR, CHNG1, LGR3, hTSHR-I, thyrotropin receptor
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20- 1:50

Format

Calculated MW

28kDa/30kDa/86kDa

Observed MW

110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-253 of human TSHR (NP_001018046.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TSHR antibody (144-06781) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - TSHR Polyclonal Antibody (144-06781)

Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (144-06781) at dilution of 1:100 (40x lens).
Immunohistochemistry - TSHR Polyclonal Antibody (144-06781)

Immunohistochemistry of paraffin-embedded mouse brain using TSHR Antibody (144-06781) at dilution of 1:100 (40x lens).
Immunohistochemistry - TSHR Polyclonal Antibody (144-06781)

Expiration:

12 months from the date of shipment when stored properly.

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