Anti-TSN (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q15631
Gene Id	7247
Gene Symbols	TSN
Protein Name / Synonyms	TSN (Center)
Clonality	Polyclonal
Preparation	This TSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human TSN.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms.

Specificity

Predicted Reactivity

Bovine, Hamster

Other Names

TSN; Translin; Component 3 of promoter of RISC

NCBI Accession #

NP_001248330.1;NP_004613.1

Other Accession #

Q62348; P97891; Q08DM8; NP_004613.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

26183

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50

	TSN Antibody (Center) (Cat# 102-11532) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).
	Fluorescent confocal image of Hela cell stained with TSN Antibody(Center)(Cat# 102-11532).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.
	TSN Antibody (Center) (Cat# 102-11532)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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