RayBiotech

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ISO 13485:2016 cGMP

Anti-TSPAN8 Antibody

Catalog #: 144-60256

TSPAN8 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesMouse, Rat
Host SpeciesRabbit
Accession Number
P19075
Gene Id
7103
Gene Symbols
TSPAN8
Protein Name / Synonyms
TSPAN8, CO-029, TM4SF3, tetraspanin-8
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
26kDa
Observed MW
26kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 110-200 of human TSPAN8 (NP_004607.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TSPAN8 antibody (144-60256) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - TSPAN8 antibody (144-60256)

Expiration:
12 months from the date of shipment when stored properly.