Anti-TST Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q16762
Gene Id	7263
Gene Symbols	TST
Protein Name / Synonyms	TST, RDS, thiosulfate sulfurtransferase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:1000 - 1:2000
IP 1:50 - 1:100

Format

Calculated MW

33kDa

Observed MW

36kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TST antibody (144-010542) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - TST Polyclonal Antibody (144-010542)

Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody (144-010542). Western blot was performed from the immunoprecipitate using TST antibody (144-010542) at a dilition of 1:1000.
Immunoprecipitation - TST Polyclonal Antibody (144-010542)

Expiration:

12 months from the date of shipment when stored properly.

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