RayBiotech

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ISO 13485:2016 cGMP

Anti-TTF2 Antibody

Catalog #: 144-07751

TTF2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q9UNY4
Gene Id
8458
Gene Symbols
TTF2
Protein Name / Synonyms
TTF2, HuF2, ZGRF6, transcription termination factor 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
75kDa/129kDa
Observed MW
130kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTF2 (NP_003585.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TTF2 antibody (144-07751) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - TTF2 antibody (144-07751)

Immunofluorescence analysis of A549 cells using TTF2 antibody (144-07751). Blue: DAPI for nuclear staining.
Immunofluorescence - TTF2 antibody (144-07751)

Expiration:
12 months from the date of shipment when stored properly.