Anti-TUBB3 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q13509
Gene Id	10381
Gene Symbols	TUBB3
Protein Name / Synonyms	TUBB3 (N-term)
Clonality	Polyclonal
Preparation	This TUBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human TUBB3.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

Specificity

Predicted Reactivity

Rat, Bovine, Chicken, Monkey

Other Names

TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

NCBI Accession #

NP_001184110.1;NP_006077.2

Other Accession #

P09652; Q4QRB4; Q9ERD7; Q60HC2; Q2T9S0; NP_006077.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

50433

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	TUBB3 Antibody (N-term) (Cat# 102-16571) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).
	TUBB3 Antibody (N-term) (Cat# 102-16571) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).
	TUBB3 Antibody (N-term) (Cat# 102-16571) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TUBB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	TUBB3 Antibody (N-term) (Cat# 102-16571) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products