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Anti-TUBB8 Antibody
Catalog #: 144-12617
TUBB8 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q3ZCM7 |
| Gene Id | 347688 |
| Gene Symbols | TUBB8 |
| Protein Name / Synonyms | TUBB8, OOMD, OOMD2, bA631M21.2, tubulin beta-8 chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene represents the primary beta-tubulin subunit of oocytes and the early embryo. Defects in this gene, which is primate-specific, are a cause of oocyte maturation defect 2 and infertility.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
49kDa
Observed MW
54kDa
Immunogen
A synthetic peptide of human TUBB8
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TUBB8 antibody (144-12617) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human stomach using TUBB8 antibody (144-12617) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using TUBB8 antibody (144-12617) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TUBB8 antibody (144-12617) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.