RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-TXN Antibody

Catalog #: 144-07638

TXN Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P10599
Gene Id
7295
Gene Symbols
TXN
Protein Name / Synonyms
TXN, TRDX, TRX, TRX1, thioredoxin
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IP
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50

Format

Calculated MW
9kDa/11kDa
Observed MW
12kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human TXN (NP_003320.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TXN antibody (144-07638) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - TXN Polyclonal Antibody (144-07638)

Immunohistochemistry of paraffin-embedded mouse lung using TXN antibody (144-07638) at dilution of 1:100 (40x lens).
Immunohistochemistry - TXN Polyclonal Antibody (144-07638)

Expiration:
12 months from the date of shipment when stored properly.