Anti-TYR Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P14679
Gene Id	7299
Gene Symbols	TYR
Protein Name / Synonyms	TYR, ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3, tyrosinase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

42kDa/60kDa

Observed MW

70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-340 of human TYR (NP_000363.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TYR antibody (144-01254) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - TYR Polyclonal Antibody (144-01254)

Immunohistochemistry of paraffin-embedded rat heart using TYR antibody (144-01254) at dilution of 1:100 (40x lens).
Immunohistochemistry - TYR Polyclonal Antibody (144-01254)

Immunofluorescence analysis of U2OS cells using TYR antibody (144-01254). Blue: DAPI for nuclear staining.
Immunofluorescence - TYR Polyclonal Antibody (144-01254)

Expiration:

12 months from the date of shipment when stored properly.

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