RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-UBA5 Antibody

Catalog #: 144-61090

UBA5 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9GZZ9
Gene Id
79876
Gene Symbols
UBA5
Protein Name / Synonyms
UBA5, EIEE44, SCAR24, THIFP1, UBE1DC1, ubiquitin-like modifier-activating enzyme 5
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW
38kDa/44kDa
Observed MW
50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human UBA5 (NP_079094.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UBA5 antibody (144-015514) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - UBA5 antibody (144-015514)

Expiration:
12 months from the date of shipment when stored properly.