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Anti-UBA6 Antibody
Catalog #: 144-07511
UBA6 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | A0AVT1 |
| Gene Id | 55236 |
| Gene Symbols | UBA6 |
| Protein Name / Synonyms | UBA6, E1-L2, MOP-4, UBE1L2, ubiquitin-like modifier-activating enzyme 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
37kDa/43kDa/65kDa/117kDa
Observed MW
59kDa, 118kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UBA6 antibody (144-07511) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human kidney using UBA6 antibody (144-07511) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBA6 antibody (144-07511). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug UBA6 antibody (144-07511). Western blot was performed from the immunoprecipitate using UBA6 antibody (144-07511) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.