Anti-UBASH3B Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8TF42
Gene Id	84959
Gene Symbols	UBASH3B
Protein Name / Synonyms	UBASH3B, STS-1, STS1, TULA-2, TULA2, p70, ubiquitin associated and SH3 domain containing B
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

72kDa

Observed MW

73kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human UBASH3B (NP_116262.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UBASH3B antibody (144-07141) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - UBASH3B antibody (144-07141)

Immunohistochemistry of paraffin-embedded rat spleen using UBASH3B antibody (144-07141) at dilution of 1:100 (40x lens).
Immunohistochemistry - UBASH3B antibody (144-07141)

Immunofluorescence analysis of A549 cells using UBASH3B antibody (144-07141). Blue: DAPI for nuclear staining.
Immunofluorescence - UBASH3B antibody (144-07141)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products