Anti-UBE1C (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q8TBC4
Gene Id	9039
Gene Symbols	UBA3
Protein Name / Synonyms	UBE1C (C-term)
Clonality	Polyclonal
Preparation	This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-445 amino acids from the C-terminal region of human UBE1C.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Specificity

Predicted Reactivity

Rat, Zebrafish

Other Names

UBA3; UBE1C; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3

NCBI Accession #

NP_003959.3;NP_937838.1

Other Accession #

Q99MI7; Q8C878; Q7ZVX6

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

51852

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-UBE1 C-term Pab (Cat# 102-10727) is used in Western blot to detect UBE1 in mouse brain tissue lysate.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	UBE1C Antibody (S430) (Cat# 102-10727) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).
	Western blot analysis of UBE1C (arrow) using rabbit polyclonal UBE1C Antibody (S430) (Cat# 102-10727). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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