UBE2C Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O00762 |
| Gene Id | 11065 |
| Gene Symbols | UBE2C |
| Protein Name / Synonyms | UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2 C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
Format
Calculated MW
15kDa/16kDa/17kDa/19kDa
Observed MW
20kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UBE2C antibody (144-05499) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using UBE2C antibody (144-05499) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using UBE2C antibody (144-05499) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using UBE2C antibody (144-05499). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 150ug extracts of SW620 cells using 3ug UBE2C antibody (144-05499). Western blot was performed from the immunoprecipitate using UBE2C antibody (144-05499) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.
