Anti-UBE2H Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P62256
Gene Id	7328
Gene Symbols	UBE2H
Protein Name / Synonyms	UBE2H, E2-20K, GID3, UBC8, UBCH, UBCH2, ubiquitin-conjugating enzyme E2 H
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

17kDa/20kDa

Observed MW

21kDa, 42kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2H (NP_003335.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UBE2H antibody (144-07344) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - UBE2H antibody (144-07344)

Immunofluorescence analysis of A549 cells using UBE2H antibody (144-07344). Blue: DAPI for nuclear staining.
Immunofluorescence - UBE2H antibody (144-07344)

Expiration:

12 months from the date of shipment when stored properly.

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