Anti-UBE2I Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P63279
Gene Id	7329
Gene Symbols	UBE2I
Protein Name / Synonyms	UBE2I, C358B7.1, P18, UBC9, SUMO-conjugating enzyme UBC9
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

18kDa

Observed MW

18kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UBE2I antibody (144-02193) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - UBE2I Polyclonal Antibody (144-02193)

Immunohistochemistry of paraffin-embedded rat brain using UBE2I antibody (144-02193) at dilution of 1:100 (40x lens).
Immunohistochemistry - UBE2I Polyclonal Antibody (144-02193)

Immunohistochemistry of paraffin-embedded mouse kidney using UBE2I antibody (144-02193) at dilution of 1:100 (40x lens).
Immunohistochemistry - UBE2I Polyclonal Antibody (144-02193)

Expiration:

12 months from the date of shipment when stored properly.

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