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Anti-UBE2L3 Antibody
Catalog #: 144-04175
UBE2L3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P68036 |
| Gene Id | 7332 |
| Gene Symbols | UBE2L3 |
| Protein Name / Synonyms | UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4, ubiquitin-conjugating enzyme E2 L3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:1000 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
14kDa/17kDa/24kDa
Observed MW
18kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_001243284.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UBE2L3 antibody (144-04175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat heart using UBE2L3 antibody (144-04175) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using UBE2L3 antibody (144-04175) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using UBE2L3 antibody (144-04175) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.