Anti-UBE2O Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9C0C9
Gene Id	63893
Gene Symbols	UBE2O
Protein Name / Synonyms	UBE2O, E2-230K, ubiquitin conjugating enzyme E2 O
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Dilutions

WB 1:1000 - 1:4000
IP 1:50 - 1:200

Format

Calculated MW

141kDa

Observed MW

200kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UBE2O antibody (144-10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] UBE2O Polyclonal Antibody (144-10036)

Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody (144-10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - [KO Validated] UBE2O Polyclonal Antibody (144-10036)

Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody (144-10036). Western blot was performed from the immunoprecipitate using UBE2O antibody (144-10036) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] UBE2O Polyclonal Antibody (144-10036)

Expiration:

12 months from the date of shipment when stored properly.

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