Anti-UBE2S Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q16763
Gene Id	27338
Gene Symbols	UBE2S
Protein Name / Synonyms	UBE2S, E2-EPF, E2EPF, EPF5, ubiquitin-conjugating enzyme E2 S
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

Dilutions

WB 1:200 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

23kDa

Observed MW

24kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 122-221 of human UBE2S (NP_055316.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UBE2S antibody (144-04658) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - UBE2S antibody (144-04658)

Immunohistochemistry of paraffin-embedded human colon carcinoma using UBE2S antibody (144-04658) at dilution of 1:100 (40x lens).
Immunohistochemistry - UBE2S antibody (144-04658)

Immunohistochemistry of paraffin-embedded human prostate cancer using UBE2S antibody (144-04658) at dilution of 1:100 (40x lens).
Immunohistochemistry - UBE2S antibody (144-04658)

Immunohistochemistry of paraffin-embedded human stomach using UBE2S antibody (144-04658) at dilution of 1:100 (40x lens).
Immunohistochemistry - UBE2S antibody (144-04658)

Expiration:

12 months from the date of shipment when stored properly.

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