Anti-UBE2Z Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9H832
Gene Id	65264
Gene Symbols	UBE2Z
Protein Name / Synonyms	UBE2Z, HOYS7, USE1, ubiquitin-conjugating enzyme E2 Z
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an enzyme which ubiquitinates proteins which participate in signaling pathways and apoptosis.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

28kDa/38kDa

Observed MW

25kDa, 39kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 115-354 of human UBE2Z (NP_075567.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UBE2Z antibody (144-07225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - UBE2Z Polyclonal Antibody (144-07225)

Immunohistochemistry of paraffin-embedded human kidney using UBE2Z antibody (144-07225) at dilution of 1:100 (40x lens).
Immunohistochemistry - UBE2Z Polyclonal Antibody (144-07225)

Immunohistochemistry of paraffin-embedded mouse kidney using UBE2Z antibody (144-07225) at dilution of 1:100 (40x lens).
Immunohistochemistry - UBE2Z Polyclonal Antibody (144-07225)

Immunofluorescence analysis of A-549 cells using UBE2Z antibody (144-07225). Blue: DAPI for nuclear staining.
Immunofluorescence - UBE2Z Polyclonal Antibody (144-07225)

Expiration:

12 months from the date of shipment when stored properly.

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