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Anti-UBQLN2 Antibody

Catalog #: 144-09568

UBQLN2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q9UHD9
Gene Id
29978
Gene Symbols
UBQLN2
Protein Name / Synonyms
UBQLN2, ALS15, CHAP1, DSK2, HRIHFB2157, N4BP4, PLIC2, ubiquilin-2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaAutophagy
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW
65kDa
Observed MW
66kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human UBQLN2 (NP_038472.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UBQLN2 antibody (144-09568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - UBQLN2 antibody (144-09568)

Expiration:
12 months from the date of shipment when stored properly.