Anti-UCN2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q96RP3
Gene Id	90226
Gene Symbols	UCN2
Protein Name / Synonyms	UCN2, SRP, UCN-II, UCNI, UR, URP, urocortin-2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Format

Calculated MW

12kDa

Observed MW

12kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-112 of human UCN2 (NP_149976.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of rat skin, using UCN2 antibody (144-06485) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - UCN2 antibody (144-06485)

Immunohistochemistry of paraffin-embedded mouse heart using UCN2 antibody (144-06485) at dilution of 1:100 (40x lens).
Immunohistochemistry - UCN2 antibody (144-06485)

Immunofluorescence analysis of U2OS cells using UCN2 antibody (144-06485). Blue: DAPI for nuclear staining.
Immunofluorescence - UCN2 antibody (144-06485)

Expiration:

12 months from the date of shipment when stored properly.

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