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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O60701 |
| Gene Id | 7358 |
| Gene Symbols | UGDH |
| Protein Name / Synonyms | UGDH, GDH, UDP-GlcDH, UDPGDH, UGD, UDP-glucose 6-dehydrogenase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
44kDa/47kDa/55kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 165-494 of human UGDH (NP_003350.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UGDH Antibody (144-01210) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat brain using UGDH antibody (144-01210) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using UGDH antibody (144-01210) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using UGDH antibody (144-01210). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.