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Anti-UHRF1 Antibody
Catalog #: 144-02343
UHRF1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q96T88 |
| Gene Id | 29128 |
| Gene Symbols | UHRF1 |
| Protein Name / Synonyms | UHRF1, ICBP90, Np95, RNF106, TDRD22, hNP95, hUHRF1, huNp95, E3 ubiquitin-protein ligase UHRF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:20 - 1:50
Format
Calculated MW
89kDa/91kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UHRF1 (NP_001041666.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using UHRF1 antibody (144-02343) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human tonsil using UHRF1 antibody (144-02343) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using UHRF1 antibody (144-02343) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using UHRF1 antibody (144-02343) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using UHRF1 antibody (144-02343) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using UHRF1 antibody (144-02343) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using UHRF1 antibody (144-02343). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.