Anti-UPF1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q92900
Gene Id	5976
Gene Symbols	UPF1
Protein Name / Synonyms	UPF1, HUPF1, NORF1, RENT1, pNORF1, smg-2, UPF1, RNA helicase and ATPase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

123kDa/124kDa

Observed MW

130kDa

Immunogen

A synthetic peptide of human UPF1

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UPF1 antibody (144-01521) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - UPF1 antibody (144-01521)

Immunofluorescence analysis of HeLa cells using UPF1 antibody (144-01521). Blue: DAPI for nuclear staining.
Immunofluorescence - UPF1 antibody (144-01521)

Expiration:

12 months from the date of shipment when stored properly.

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