RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-UQCRC2 Antibody

Catalog #: 144-04181

UQCRC2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P22695
Gene Id
7385
Gene Symbols
UQCRC2
Protein Name / Synonyms
UQCRC2, MC3DN5, QCR2, UQCR2, cytochrome b-c1 complex subunit 2, mitochondrial
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IP
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is located in the mitochondrion, where it is part of the ubiquinol-cytochrome c reductase complex (also known as complex III). This complex constitutes a part of the mitochondrial respiratory chain. Defects in this gene are a cause of mitochondrial complex III deficiency nuclear type 5.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW
48kDa
Observed MW
48kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 224-453 of human UQCRC2 (NP_003357.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using UQCRC2 antibody (144-04181) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - UQCRC2 Polyclonal Antibody (144-04181)

Western blot analysis of extracts of various cells, using UQCRC2 antibody (144-04181) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - UQCRC2 Polyclonal Antibody (144-04181)

Immunohistochemistry of paraffin-embedded human liver injury using UQCRC2 antibody (144-04181) at dilution of 1:100 (40x lens).
Immunohistochemistry - UQCRC2 Polyclonal Antibody (144-04181)

Expiration:
12 months from the date of shipment when stored properly.