Anti-USF1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P22415
Gene Id	7391
Gene Symbols	USF1
Protein Name / Synonyms	USF1, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, bHLHb11, upstream stimulatory factor 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

27kDa/33kDa

Observed MW

41kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human USF1 (NP_009053.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse kidney, using USF1 antibody (144-60393) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - USF1 antibody (144-60393)

Immunohistochemistry of paraffin-embedded human kidney cancer using USF1 antibody (144-60393) at dilution of 1:200 (40x lens).
Immunohistochemistry - USF1 antibody (144-60393)

Immunohistochemistry of paraffin-embedded mouse lung using USF1 antibody (144-60393) at dilution of 1:200 (40x lens).
Immunohistochemistry - USF1 antibody (144-60393)

Expiration:

12 months from the date of shipment when stored properly.

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