RayBiotech

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ISO 13485:2016 cGMP

Anti-USP1 Antibody

Catalog #: 144-06785

USP1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
O94782
Gene Id
7398
Gene Symbols
USP1
Protein Name / Synonyms
USP1, UBP, ubiquitin specific peptidase 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
88kDa
Observed MW
110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 486-785 of human USP1 (NP_003359.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using USP1 Antibody (144-06785) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - USP1 Antibody (144-06785)

Expiration:
12 months from the date of shipment when stored properly.