Anti-USP13 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q92995
Gene Id	8975
Gene Symbols	USP13
Protein Name / Synonyms	USP13 (Center)
Clonality	Polyclonal
Preparation	This USP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-419 amino acids from the Central region of human USP13.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Specificity

Predicted Reactivity

Bovine

Other Names

USP13; ISOT3; Ubiquitin carboxyl-terminal hydrolase 13; Deubiquitinating enzyme 13; Isopeptidase T-3; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13

NCBI Accession #

NP_003931.2

Other Accession #

Q5BKP2; E1BMF7; NP_003931

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

97327

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-USP13 Pab (Cat# 102-14994) is used in Western blot to detect USP13 in HeLa cell lysate.
	Detection of USP13 in HeLa cells expressing exogenous USP13 by anti-USP13 Pab (Cat# 102-14994). (Data provided by Amy Chen, Burnham Institute for Medical Research)
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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