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Anti-USP7 Antibody
Catalog #: 144-60394
USP7 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q93009 |
| Gene Id | 7874 |
| Gene Symbols | USP7 |
| Protein Name / Synonyms | USP7, HAUSP, TEF1, ubiquitin specific peptidase 7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
126kDa/128kDa
Observed MW
138kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 206-1102 of human USP7 (NP_003461.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using USP7 antibody (144-60394) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human kidney cancer using USP7 antibody (144-60394) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using USP7 antibody (144-60394) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using USP7 antibody (144-60394).
Expiration:
12 months from the date of shipment when stored properly.